Integrating Cell Biology, Image Analysis, and
Computational Mechanical Modeling to Analyze the Contributions of
Cellulose and Xyloglucan to Stomatal Function
Yue Rui, Hojae Yi, Baris Kandemir, James Z. Wang,
Virendra M. Puri, and Charles T. Anderson
The Pennsylvania State University
Abstract:
Cell walls are likely to be essential determinants of the amazing
strength and flexibility of the guard cells that surround each
stomatal pore in plants, but surprisingly little is known about cell
wall composition, organization, and dynamics in guard cells. Recent
analyses of cell wall organization and stomatal function in the guard
cells of Arabidopsis thaliana mutants with defects in cellulose and
xyloglucan have allowed for the development of new hypotheses about
the relative contributions of these components to guard cell
function. Advanced image analysis methods can allow for the automated
detection of key structures, such as microtubules (MTs) and Cellulose
Synthesis Complexes (CSCs), in guard cells, to help determine their
contributions to stomatal function. A major challenge in the
mechanical modeling of dynamic biological structures, such as guard
cell walls, is to connect nanoscale features (e.g., wall polymers and
their molecular interactions) with cell-scale mechanics; this
challenge can be addressed by applying multiscale computational
modeling that spans multiple spatial scales and physical attributes
for cell walls.
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Citation:
Yue Rui, Hojae Yi, Baris Kandemir, James Z. Wang, Virendra M. Puri and
Charles T. Anderson, ``Integrating Cell Biology, Image Analysis, and
Computational Mechanical Modeling to Analyze the Contributions of
Cellulose and Xyloglucan to Stomatal Function,'' Plant Signaling &
Behavior, vol. 11, no. 6, e1183086:1-4, 2016.
© 2016 Taylor & Francis. This is an Accepted Manuscript of an
article published by Taylor & Francis in Plant Signaling &
Behavior on 24/05/2016,
available online: http://www.tandfonline.com/doi/full/10.1080/15592324.2016.1183086
Last Modified:
May 26, 2016
© 2016